P-18: Protective Effect of Selenium- Enriched Saccharomyces Cerevisiae Cytoplasm and Cell Wall on Chronic Immobilization Stress-Induced Damages in Testis; Evidence for Apoptosis

Background Previous reports showed that immobilization stress (IMS) results in severe damages at spermatogenesis level. Present study was performed in order to evaluate the protective effect of selenium-enriched yeast fragments on IMS-induced derangements. MaterialsAndMethods For this purpose, 42 mature male Wister rats were assigned into 6 groups (7 rats in each group) including; control, stress-induced and SE-enriched saccharomyces cerevisiae fragments-treated groups. The animals in treatment groups subdivided into 4 groups as; SE-enriched yeast cytoplasm (SEC)+stress, SE-enriched saccharomyces cerevisiae cell wall+stress (SECW), SEC alone-treated and SECW alone-treated groups. In order to induce stress, the rats were immobilized by keeping them into transparent plastic jars with 5 holes for 2 hours a day. All animals received the chemicals (5×108 CFU/ml, in one mL for day) orally for 42 days. At the end of day 42. The testicles were dissected out, fixed in formaldehyde. The expression of bcl-2 and p53 were investigated by using immnohistochemical and RT-PCR techniques. Moreover, the TUNEL staining was performed in order to evaluate apoptosis. Results Observations demonstrated that co-administrating cytoplasm+cell wall in SECW group significantly (P